Lung transplantation (LTx) is required for patients with end-stage lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis. Ischemia-reperfusion injury (IRI) can occur due to the loss and then subsequent restoration of blood flow that occurs to organs during the transplant procedure. This can induce the development of primary graft dysfunction (PGD) which is a lung injury that occurs within the first 72 hours post-LTx. Current biomarkers of PGD are limited to inflammatory cytokines that are a component of the complement activation cascade. We are investigating the antibody immunoglobulin G (IgG)’s role in the activation of the complement immune response by analyzing IgG’s glycosylation in biofluids utilizing imaging mass spectrometry (IMS). We also utilize IMS to analyze mouse models of PGD post-LTx to generate a spatial atlas of the N-glycome to study the development and progression of PGD.